anti cd47 antibody  (Bio X Cell)

Journal: Cell Death & Disease

Article Title: Macrophages rescue cells from ferroptotic death

doi: 10.1038/s41419-025-08277-6

Figure Lengend Snippet: a CD47 expression before (control) and 2 h and 5 h after ferroptosis (RSL3) or apoptosis (Staurosporine) induction in BeWo and A375 cells. Graphs show an average (± STD) CD47 median fluorescence intensity (MFI) of 4 replicates in each group. b The indicated cell types were treated with RSL3 (500 nM) for 1 h, followed by preincubation with either blocking anti-human CD47 antibodies (5 µg/ml) or IgG1κ isotype control antibodies (5 µg/ml) for 15 min. Subsequently, Far-red-labeled THP-1-derived macrophages were added. Cell survival was assessed by flow cytometry as described above. Similar results were obtained when 10 and 20 µg/ml blocking antibody was used. Graphs display an average (±STD) of 6 replicates in each group. c Cells were treated as in ( a ) and were then stained with annexin V and PI. The percentage of annexin-positive cells is shown. Graphs display an average (±STD) of 4 replicates in each group. Representative of at least three independent experiments is shown. ns non-significant; *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001.

Article Snippet: Additionally, in several experiments, cells were pre-incubated for 15 min with either anti-human CD47 antibodies (5–20 μg/ml; Clone B6.H12; Cat. No. BE0019-1, Bio X Cell) or isotype control antibodies (5–20 μg/ml; mouse IgG1κ; Cat. No. 400102, BioLegend), after which macrophages were added into the co-culture.

Techniques: Expressing, Control, Fluorescence, Blocking Assay, Labeling, Derivative Assay, Flow Cytometry, Staining